核酸酶
重组DNA
粘质沙雷氏菌
化学
基因亚型
分子质量
大肠杆菌
分子生物学
质谱法
生物化学
肽序列
基因
生物
酶
色谱法
作者
Jacob Krabbe Pedersen,Gitte Brinch Andersen,Peter Roepstorff,Maria Filimonova,K Bidermann
出处
期刊:PubMed
日期:1995-05-01
卷期号:21 (5): 330-5
被引量:1
摘要
Isoforms of the natural and recombinant nucleases of Serratia marcescens were characterized by their molecular mass, which was determined by electrospray mass spectrometry. The natural nuclease was isolated from the S. marcescens B10M1 culture, whereas the recombinant nuclease was obtained from Escherichia coli MT102 cells carrying plasmid p403-SD2 with the nuclease gene nuc. The primary structure for each of the isoforms isolated from the nuclease preparations was determined by comparing its molecular mass with that of known amino acid sequence, which was determined from the nucleotide sequence of the nuc gene. Both preparations included identical nuclease variants with N-terminal amino acid residues removed. The number of isoforms in the natural nuclease was, however, significantly greater than in the recombinant nuclease. The structures of some of the isoforms were confirmed by N-terminal analysis.
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