生物标志物
导管内乳头状粘液性肿瘤
医学
胰腺导管腺癌
生物标志物发现
胰腺癌
内科学
肿瘤科
病理
癌症
蛋白质组学
胰腺
化学
生物化学
基因
作者
Yikwon Kim,Minjung Kang,Dohyun Han,Hyunsoo Kim,KyoungBun Lee,Sun‐Whe Kim,Yongkang Kim,Taesung Park,Jin–Young Jang,Youngsoo Kim
标识
DOI:10.1021/acs.jproteome.5b00553
摘要
Intraductal papillary mucinous neoplasm (IPMN) is a common precursor of pancreatic cancer (PC). Much clinical attention has been directed toward IPMNs due to the increase in the prevalence of PC. The diagnosis of IPMN depends primarily on a radiological examination, but the diagnostic accuracy of this tool is not satisfactory, necessitating the development of accurate diagnostic biomarkers for IPMN to prevent PC. Recently, high-throughput targeted proteomic quantification methods have accelerated the discovery of biomarkers, rendering them powerful platforms for the evolution of IPMN diagnostic biomarkers. In this study, a robust multiple reaction monitoring (MRM) pipeline was applied to discovery and verify IPMN biomarker candidates in a large cohort of plasma samples. Through highly reproducible MRM assays and a stringent statistical analysis, 11 proteins were selected as IPMN marker candidates with high confidence in 184 plasma samples, comprising a training (n = 84) and test set (n = 100). To improve the discriminatory power, we constructed a six-protein panel by combining marker candidates. The multimarker panel had high discriminatory power in distinguishing between IPMN and controls, including other benign diseases. Consequently, the diagnostic accuracy of IPMN can be improved dramatically with this novel plasma-based panel in combination with a radiological examination.
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