免疫沉淀
染色质免疫沉淀
组蛋白
计算生物学
仿形(计算机编程)
DNA甲基化
表观遗传学
背景(考古学)
生物
炸薯条
DNA
计算机科学
细胞生物学
遗传学
基因
基因表达
发起人
古生物学
电信
操作系统
作者
Julie Brind'Amour,Matthew C Lorincz
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 229-251
标识
DOI:10.1007/978-1-0716-2481-4_11
摘要
AbstractChromatin immunoprecipitation (ChIP) enables the study of DNA–protein interactions. When coupled with high-throughput sequencing (ChIP-seq), this method allows the generation of genome-wide profiles of the distribution of specific proteins in a given cellular context. Typical ChIP-seq experiments require millions of cells as input material and thus are not ideal to study many in vivo cell populations. Here, we describe an ultra-low-input native ChIP-seq method, ULI-NChIP-seq, to profile histone modification patterns in as low as 150 cells.Key wordsChromatinHistone modificationsEpigeneticsLow-inputEmbryoOocyteMethylationChromatin immunoprecipitation
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