草酸钙
乙醛酸循环
腹腔注射
草酸盐
医学
内科学
肾
内分泌学
骨桥蛋白
尿
钙
乙二醇
尿酸
化学
新陈代谢
无机化学
有机化学
作者
Seung Young Oh,Jong Kyou Kwon,Seo Yeon Lee,Moon Soo Ha,Yong Uk Kwon,Young Tae Moon
出处
期刊:Journal of Endourology
[Mary Ann Liebert]
日期:2011-06-01
卷期号:25 (6): 1057-1061
被引量:6
标识
DOI:10.1089/end.2010.0386
摘要
Purpose: The aim of this study was to design a simple and convenient rat model with significant calcium oxalate crystal deposition in the kidney. Materials and Methods: Sprague-Dawley rats were divided into seven groups of 30 rats each. One group of rats was untreated to serve as controls. Two of the groups of rats were provided with drinking water supplemented with 1% ethylene glycol (EG), and of these two groups, one group was also administered vitamin D. Two groups of rats received intraperitoneal injections of EG, and of these two groups, one group also received vitamin D. Two groups of rats received intraperitoneal injections of glyoxylate, and of these two groups, one group also received vitamin D. We analyzed 24-hour urine samples for urinary constituents for all experimental groups, including calcium, oxalate, citrate, uric acid, pH, and urine volume. The kidneys were examined for crystal deposition using histologic examination and for osteopontin expression using immunohistochemical staining. Results: Calcium-oxalate crystals were found in all rats injected with glyoxylate after 1 week. The degree of crystal deposition in rats injected with glyoxylate for 1 week was significantly increased compared with rats fed EG for 4 weeks. Conclusions: Intraperitoneal injection of glyoxylate is a faster, more exact, and more reliable method to produce calcium oxalate crystal deposition in the kidney than previous urolithiasis animal models.
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