同源盒蛋白纳米
胰腺癌
癌症干细胞
癌症研究
基因敲除
SOX2
纳米同源盒蛋白
生物
转移
癌变
干细胞
胚胎干细胞
癌症
细胞培养
细胞生物学
诱导多能干细胞
遗传学
基因
作者
Yao Lu,Hui Zhu,Haiyan Shan,Junjie Lu,Chong Xu,LI Xiao-hong,Jingjing Lu,Xiaoxuan Fan,Shajun Zhu,Yao Wang,Qingsong Guo,Lei Wang,Yan Huang,Mingyan Zhu,Zhiwei Wang
出处
期刊:Cancer Letters
[Elsevier]
日期:2013-10-01
卷期号:340 (1): 113-123
被引量:128
标识
DOI:10.1016/j.canlet.2013.07.009
摘要
Pancreatic cancer is notorious for its difficult diagnosis at early stage and poor recurrence-free prognosis. This study aimed to investigate the possible involvement of Oct4 and Nanog in pancreatic cancer. The high expressions of Oct4 and Nanog in human pancreatic cancer tissues were found to indicate a worse prognostic value of patients. The pancreatic cancer stem cells (PCSCs) that isolated from PANC-1 cell line by flow cytometry exhibited high expressions of Oct4 and Nanog. To investigate whether Oct4 and Nanog play crucial role in maintaining the stemness of PCSCs, double knockdown of Oct4 and Nanog demonstrated that Oct4 and Nanog significantly reduced proliferation, migration, invasion, chemoresistance, and tumorigenesis of PCSCs in vitro and in vivo. The altered expression of the genes related to pancreatic carcinogenesis, metastasis, drug resistance and epithelial-mesenchymal transdifferentiation (EMT) might affect the biological characteristics of PCSCs. Our results suggest that Oct4 and Nanog may serve as a potential marker of prognosis and a novel target of therapy for pancreatic cancer.
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