Determination of glucosinolate in rapeseed meal by infrared spectrometry

A method is proposed for the determination of glucosinolate in rapeseed meal by infrared spectrometry. Glucosinolate has a strong absorption peak at 800 cm–1. As rapeseed oil and meal do not absorb at 800 cm–1, they do not interfere in the determination at this wavelength. The relationship between the absorption intensity and concentration of glucosinolate obeyed Beer's law in the range 0.0–43.8 µg. A concentration of 2.4–4.8 µmol of glucosinolate per gram of meal can be detected in this way. One quarter to half of a grain of rapeseed is sufficient to determine glucosinolate directly.