荧光
光化学
活体细胞成像
纳米技术
化学
荧光显微镜
材料科学
生物物理学
光学
细胞
物理
生物
生物化学
作者
Bingjie Zhao,Daoming Guan,Jinyang Liu,Xuebo Zhang,Shuzhang Xiao,Yunxiang Zhang,Bradley D. Smith,Qian Liu
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-04-08
标识
DOI:10.1021/acs.nanolett.4c00595
摘要
Hampered by their susceptibility to nucleophilic attack and chemical bleaching, electron-deficient squaraine dyes have long been considered unsuitable for biological imaging. This study unveils a surprising twist: in aqueous environments, bleaching is not irreversible but rather a reversible spontaneous quenching process. Leveraging this new discovery, we introduce a novel deep-red squaraine probe tailored for live-cell super-resolution imaging. This probe enables single-molecule localization microscopy (SMLM) under physiological conditions without harmful additives or intense lasers and exhibits spontaneous blinking orchestrated by biological nucleophiles, such as glutathione or hydroxide anion. With a low duty cycle (∼0.1%) and high-emission rate (∼6 × 104 photons/s under 400 W/cm2), the squaraine probe surpasses the benchmark Cy5 dye by 4-fold and Si-rhodamine by a factor of 1.7 times. Live-cell SMLM with the probe reveals intricate structural details of cell membranes, which demonstrates the high potential of squaraine dyes for next-generation super-resolution imaging.
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