清脆的
生物
反式激活crRNA
质粒
原噬菌体
流动遗传元素
Cas9
遗传学
计算生物学
基因组
核糖核酸
基因
背景(考古学)
DNA
CRISPR干扰
噬菌体
大肠杆菌
古生物学
作者
Sarah Camara-Wilpert,David Mayo-Muñoz,Jakob Russel,Robert D. Fagerlund,Jonas Stenløkke Madsen,Peter C. Fineran,Søren J. Sørensen,Rafael Pinilla‐Redondo
出处
期刊:Nature
[Springer Nature]
日期:2023-10-18
卷期号:623 (7987): 601-607
被引量:7
标识
DOI:10.1038/s41586-023-06612-5
摘要
Many bacteria use CRISPR-Cas systems to combat mobile genetic elements, such as bacteriophages and plasmids1. In turn, these invasive elements have evolved anti-CRISPR proteins to block host immunity2,3. Here we unveil a distinct type of CRISPR-Cas Inhibition strategy that is based on small non-coding RNA anti-CRISPRs (Racrs). Racrs mimic the repeats found in CRISPR arrays and are encoded in viral genomes as solitary repeat units4. We show that a prophage-encoded Racr strongly inhibits the type I-F CRISPR-Cas system by interacting specifically with Cas6f and Cas7f, resulting in the formation of an aberrant Cas subcomplex. We identified Racr candidates for almost all CRISPR-Cas types encoded by a diverse range of viruses and plasmids, often in the genetic context of other anti-CRISPR genes5. Functional testing of nine candidates spanning the two CRISPR-Cas classes confirmed their strong immune inhibitory function. Our results demonstrate that molecular mimicry of CRISPR repeats is a widespread anti-CRISPR strategy, which opens the door to potential biotechnological applications6.
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