色谱法
一元酸
化学
代谢物
高效液相色谱法
固相萃取
检出限
萃取(化学)
氯仿
生物化学
高分子化学
作者
Joan Bergman,Lainey Harvill,Joe S. Smith,Ellen Haynes,Christopher A. Cleveland,Michael J. Yabsley,Sarah M. Coker,Wided Najahi‐Missaoui,Deborah Elder,Sherry Cox
标识
DOI:10.1002/sscp.202300065
摘要
Abstract The purpose of this study was to establish a reliable method for the quantification of flubendazole and its metabolite, 2‐aminoflubendazole, in small‐volume canine plasma samples. Following liquid extraction with chloroform, samples were separated by reverse‐phase high‐performance liquid chromatography on an XBridge C 18 4.6 × 250 mm column (5 μm). Quantification was performed using ultraviolet detection at 246 nm. A mixture of 5 mM potassium phosphate monobasic and acetonitrile (72:28) was used as the mobile phase. The standard curve ranged from 2.5 to 1000 ng/mL. Intra‐ and interassay variance for flubendazole and 2‐aminoflubendazole was less than 6%, while the recovery ranged from 91 to 101%. The lower limit of quantification was 2.5 ng/mL. This method was successfully validated and applied to the analysis of flubendazole and 2‐aminoflubendazole samples.
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