Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis

Furthermore, the PuKAN4 could physically interact with PuMYB10/PuMYB114 and did not affect the combination of MYB10/MYB114-bHLH3, as demonstrated by Y2H, pull-down and firefly luciferase complementation. Thus, the PuKAN4 should play the role of active repressor, the formation of PuKAN4-PuMYB10/PuMYB114-PubHLH3 complex inhibited pear anthocyanin biosynthesis. Our findings unveiled an activator-and-repressor feedback loop between PuMYB114/PuMYB10 and PuKAN4, which possibly balance biosynthesis activity and prevents over-accumulation of phenylpropanoids.