免疫荧光
显微镜
间接免疫荧光
化学
生物
物理
光学
遗传学
抗体
作者
Giulia Miglietta,Jessica Marinello,Giovanni Capranico
出处
期刊:Methods in Enzymology
日期:2024-01-01
标识
DOI:10.1016/bs.mie.2024.01.008
摘要
A large variety of non-B secondary structures can be formed between DNA and RNA. In this chapter, we focus on G-quadruplexes (G4) and R-loops, which can have a close structural interplay. In recent years, increasing evidence pointed to the fact that they can strongly influence each other in vivo, both having physiological and pathological roles in normal and cancer cells. Here, we detail specific and accurate methods for purification of BG4 and S9.6 antibodies, and their subsequent use in immunofluorescence microscopy, enabling single-cell analysis of extent and localization of G4s and R-loops.
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