High‐Fidelity DNA Polymerase for DNA‐Based Digital Information Storage

DNA is increasingly recognized for its superior data storage density, favorable stability, and low energy requirements, positioning it as a potential alternative for future digital information storage systems. However, the replication and transfer of information within DNA is prone to errors, primarily due to the inaccuracy during DNA synthesis. Herein, 9°N DNA polymerase is explored from Thermococcus sp. 9°N-7 for robust DNA information storage, leveraging its thermophilic characteristics and error-correcting capability to facilitate high-fidelity DNA amplification. Notably, the enzyme demonstrate great improvement in managing DNA substitution errors compared to commercial DNA polymerases, effectively addressing the shortfall in substitution error correction typically presented in coding algorithms. This distinctive fidelity and substrate specificity of 9°N DNA polymerase is attributed to specific conformational changes and interactions during the process of nucleotide incorporation. Collectively, the findings suggested that integrating high-fidelity DNA polymerase with robust coding algorithm is a viable strategy to achieve error correction in DNA data storage. This combination exhibite the potential to augment the accuracy, portability, and scalability of DNA-based information storage systems, paving the way for reliable and effective data storage.