For more than past two decades, aquaculture has been one of the most growing activities around the globe. Branchiomyces demigrans is one of the common devastating fungal pathogens, threatening several freshwater fishes and causing mortalities, leading to huge economic losses. The diameter of hyphae of B. demigrans usually ranges from 13–14 μm to 22–28 μm at the hyphal end. The wall thickness is 0.5–0.7 μm and the diameter of spore is approximately 12–17 μm. They are found in fishes with low pH, 5.8–6.5, low dissolved oxygen, and less stress.