癌症研究
黄芪
细胞凋亡
细胞毒性
传统医学
细胞毒性T细胞
细胞生长
体外
药理学
MTT法
化学
细胞培养
体内
医学
生物
癌症
细胞周期
癌细胞
作者
Ani Georgieva,Georgi Popov,Aleksandar Shkondrov,Reneta Toshkova,Ilina Krasteva,Magdalena Kondeva-Burdina,Vasil Manov
标识
DOI:10.1016/j.jep.2020.113519
摘要
Abstract Ethnopharmacological relevance Astragalus glycyphyllos L. has been extensively used in Bulgarian folk medicine as an antihypertensive, diuretic, anti-inflammatory, anti-tumour, in cases of cardiac insufficiency, renal inflammation, calculosis, etc. Aim of the study To evaluate the possible in vitro/in vivo anti-proliferative/anti-tumour activity of a purified saponins’ mixture (PSM) obtained from the plant. Materials and methods Viability and proliferative activity of the Graffi myeloid tumour cells was assessed by MTT test. The morphological alterations were visualized and analysed by fluorescent microscopy after intravital double staining. An in vivo model of Graffi tumour bearing hamsters was used to examine the influence of PSM on transplantability, tumour growth, survival and mortality as well as to observe pathomorphological changes. Results Graffi tumour cells were sensitive to purified saponins’ mixture after 24 and 48 h treatment. The treatment induced a statistically significant decrease of the viability/proliferation of the Graffi tumour cells. These effects were concentration- and time-dependent. Fluorescent microscopy studies showed that these antiproliferative effects were connected to the induction of apoptosis. The in vivo study showed the presence of a stromal component, single mononuclear cells in the stroma. Multiple incorrect mitotic figures were observed in the tumour tissue from the control group. Well-formed stroma with accumulation of mononuclear cells and mitotic cells were found in the group, treated with PSM. The tumour weight was decreased in the group, treated with PMS. Conclusion The results indicate that PSM exhibited in vitro/in vivo antiproliferative/anti-tumour effects.
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