细胞凋亡
体内
聚ADP核糖聚合酶
结直肠癌
细胞色素c
DNA断裂
化学
体外
程序性细胞死亡
癌细胞
药理学
线粒体
传统医学
半胱氨酸蛋白酶
生物
癌症研究
生物化学
癌症
医学
聚合酶
DNA
生物技术
遗传学
作者
Xueyuan Tang,Xinying Yang,Youn‐Chul Kim,Sung-Yeon Kim,Back-Dong Kang,Da-Hye Choi,Okjin Kim,Won-Cheol Park,Hyun Park
出处
期刊:PubMed
日期:2012-06-01
卷期号:49 (3): 173-81
被引量:5
摘要
Colorectal cancer is one of the leading causes of death in the world. Plant-derived products have proven to be valuable sources for discovery and development of unique anticancer drugs. In this study, the inhibitory effects of ethanolic extract of Melia toosendan fruit (EMTF), a traditional medicine in the Chinese Pharmacopeia were evaluated in vitro and in vivo against colon cancer. Human colon cancer cells SW480 and murine colorectal adenocarcinoma cells CT26 were used to investigate cell proliferation. The results showed that EMTF inhibited cell proliferation of SW480 and CT26 by promoting apoptosis as indicated by nuclear chromatin condensation and DNA fragmentation. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria, EMTF induced caspase-9 activity which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage, leading the tumor cells to apoptosis. The in vivo results confirmed reduction of tumor volume and apoptotic effects and the side effects were not induced by EMTF. Therefore, EMTF may be an effective chemotherapeutic agent for colon cancer treatment.
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