氨肽酶
肝损伤
戒毒(替代医学)
体内
癌症研究
肝癌
化学
荧光寿命成像显微镜
对乙酰氨基酚
亮氨酸
下调和上调
医学
病理
肝细胞癌
药理学
荧光
药品
生物化学
生物
氨基酸
基因
替代医学
生物技术
量子力学
物理
作者
Xinyuan He,Lihong Li,Fang Yu,Wen Shi,Xiaohua Li,Huimin Ma
出处
期刊:Chemical Science
[The Royal Society of Chemistry]
日期:2017-01-01
卷期号:8 (5): 3479-3483
被引量:125
摘要
The liver, a main detoxification organ, has evolved a complex enzymatic system to respond to multiple pathological conditions, in which leucine aminopeptidase (LAP) has been reported to participate in detoxifying cisplatin in hepatoma cells and contribute to the intrinsic drug resistance. In vivo imaging of LAP activity in liver disease models is thus helpful to further understand the function of LAP in detoxification and medicine, but such an imaging approach is still lacking. Herein, we develop a selective and sensitive near-infrared fluorescent probe (HCAL) for this purpose. Using the probe, combined with confocal fluorescence imaging, we disclose the upregulations of LAP in acetaminophen-induced liver injury and tumor-bearing mice models. Supplementary acetylcysteine can suppress this upregulation, revealing that the LAP increase may be connected with a deficiency in biothiols. Moreover, HCAL has been used to image LAP in hepatoma cells, tumor tissues and xenograft tumor mice models successfully. These results demonstrate that HCAL may be a promising tool for studying the function of LAP in LAP-associated liver diseases.
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