适体
分子印迹
分子印迹聚合物
罗丹明B
荧光
纳米化学
检出限
聚合物
化学
印记(心理学)
聚合
材料科学
共轭体系
分子识别
组合化学
选择性
分子
纳米技术
色谱法
有机化学
生物化学
分子生物学
基因
生物
催化作用
光催化
物理
量子力学
作者
Lingyun Wang,Junyu Yang,Li Tang,Lianghui Luo,Chunyan Chen,Hang Gong,Changqun Cai
标识
DOI:10.1007/s00604-021-04858-2
摘要
An approach is reported based on the combination of aptamer and metal organic frameworks (MOF) to prepare a molecularly imprinted sensor that recognizes viruses with high specificity and sensitivity. Using MIL-101-NH2 as a polymer carrier, viral aptamers were introduced into the carrier surface through an amide reaction to specifically identify the target, and surface imprinting is carried out through tetraethyl silicate (TEOS) self-polymerization. The MIL-101-NH2 is also used as the reference fluorescence signal (λex/λem = 290/460 nm) and rhodamine B as the change signal (λex/λem = 550/570 nm). The ratiometric fluorescence detection and dual recognition strategy not only reduce environmental interference but also greatly improve the sensor’s anti-interference ability, the obtained imprinting factor was 5.72, and the detection limit as low as 1.8 pmol L−1. Therefore, the molecular imprinting sensor designed realizes the specific and highly sensitive identification of viruses, which provides theoretical support for the application of molecular imprinting technology in clinical diagnosis of viruses.
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