Stress-induced epinephrine levels compromise murine dermal fibroblast activity through β-adrenoceptors

Experimental DermatologyVolume 20, Issue 5 p. 413-419 Original Article Stress-induced epinephrine levels compromise murine dermal fibroblast activity through β-adrenoceptors Bruna Romana-Souza, Bruna Romana-Souza Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, Brazil Department of Animal Biology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, BrazilSearch for more papers by this authorMarcela Otranto, Marcela Otranto Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorTaís Fontoura Almeida, Taís Fontoura Almeida Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorLuis Cristóvão Porto, Luis Cristóvão Porto Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorAndréa Monte-Alto-Costa, Andréa Monte-Alto-Costa Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this author Bruna Romana-Souza, Bruna Romana-Souza Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, Brazil Department of Animal Biology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, BrazilSearch for more papers by this authorMarcela Otranto, Marcela Otranto Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorTaís Fontoura Almeida, Taís Fontoura Almeida Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorLuis Cristóvão Porto, Luis Cristóvão Porto Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this authorAndréa Monte-Alto-Costa, Andréa Monte-Alto-Costa Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, BrazilSearch for more papers by this author First published: 01 March 2011 https://doi.org/10.1111/j.1600-0625.2010.01239.xCitations: 25 Dr Andréa Monte-Alto-Costa, Department of Histology and Embryology, Rio de Janeiro State University (UERJ), Rua Professor Manoel de Abreu, 444, 3° andar, 20550-170 Rio de Janeiro, RJ, Brazil, Tel.: +55-21-2587-6509, Fax: +55-21-2587-6511, e-mails: [email protected]; [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Abstract: Stress-induced catecholamine impairs the formation of granulation tissue acting directly in fibroblast activity; however, the mechanism by which high levels of catecholamines alter the granulation tissue formation is still unclear. Thus, the aim of this study was to investigate how high levels of epinephrine compromise the activity of murine dermal fibroblasts. Dermal fibroblasts isolated from the skin of neonatal Swiss mice were preincubated with α- or β-adrenoceptor antagonists. Thereafter, cells were exposed to physiologically elevated levels of epinephrine or epinephrine plus α- or β-adrenoceptor antagonists, and fibroblast activity was evaluated. The blockade of β1- and β2-adrenoceptors reversed the increase in fibroblast proliferation, ERK 1/2 phosphorylation, myofibroblastic differentiation and the reduction of collagen deposition induced by epinephrine. In addition, the blockade of β3-adrenoceptors reversed the increase in fibroblast proliferation and nitric oxide synthesis as well as the reduction of fibroblast migration, AKT phosphorylation and active matrix metalloproteinase-2 expression induced by epinephrine. However, the blockade of α1- and α2-adrenoceptors did not alter the effects of epinephrine on the activity of murine dermal fibroblasts. In conclusion, high levels of epinephrine directly compromise the activity of neonatal mouse skin fibroblasts through the activation of β1-, β2- and β3-adrenoceptors, but not through α1- and α2-adrenoceptors. Supporting Information Figure S1. Cell migration observed at the edge of a scratch wound in confluent fibroblast cultures treated with epinephrine (E) or epinephrine plus propranolol (PP), SR-59230A (SR), or phentolamine (PT) for 24 hours. 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