颅咽管瘤
细胞角蛋白
免疫组织化学
体外
细胞培养
细胞质
病理
倍增时间
染色
分子生物学
生物
化学
医学
细胞生物学
内分泌学
遗传学
作者
Hao Liu,Liang Liu,Yong Li,Qiang Lu,Chao You,Jianguo Xu
出处
期刊:PubMed
日期:2012-03-15
卷期号:7 (8): 601-5
被引量:2
标识
DOI:10.3969/j.issn.1673-5374.2012.08.007
摘要
Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research.
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