纳米载体
透明质酸
化学
生物物理学
细胞内
PEG比率
细胞毒性
CD44细胞
乙二醇
荧光寿命成像显微镜
癌细胞
量子点
生物化学
纳米技术
荧光
体外
药物输送
材料科学
癌症
生物
有机化学
经济
物理
遗传学
量子力学
财务
作者
Yan Fu,Moon‐Sun Jang,Tepeng Wu,Jun Young Lee,Yang Li,Sang Soo Lee,Hong Yu Yang
标识
DOI:10.1016/j.carbpol.2019.115174
摘要
The use of multifunctional quantum dots (QDs) as smart nanocarriers has exhibited substantial promise for imaging, targeting and therapeutic functionalities. Here, we describe the synthesis of green-light emitting CdZnSeS/ZnS quantum dots (QDs) combined with redox-sensitive hyaluronic acid ligand (hyaluronic acid-disulfide-linked poly(ethylene glycol)-histamine-diethylenetriamine, HA-PEG(SS)-His-Diet) for the targeted intracellular delivery of protein drugs. The generation of HA-PEG(SS)-His-Diet-QD exhibits monodispersity with high quantum yield, negligible cytotoxicity and long-term stability at pH 7.4 and 5.5. These HA-PEG(SS)-His-Diet-QDs could effectively immobilize cytochrome C (CC) with high loading efficiency, enable target of CD44-overexpressing MCF-7 human breast tumor cells, and accelerate protein release under high intracellular glutathione concentration condition. The HA-PEG(SS)-His-Diet-QD act as a promising nanocarrier for enhanced endo/lysosomal escape, targeted delivery of proteins and real-time cellular imaging. In addition, CC-loaded-HA-PEG(SS)-His-Diet-QD could effectively induce the CD44-positive cancer cells apoptosis in vitro. Ultimately, this redox-sensitive and fluorescent QD-based nanocarrier has shown major promise for targeted intracellular protein transport.
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