化学
偏最小二乘回归
色谱法
化学计量学
叶酸
甲氨蝶呤
荧光
检出限
定量分析(化学)
反叶绿体
有机化学
毒性
统计
物理
数学
量子力学
抗代谢物
内科学
免疫学
生物
医学
作者
M. Alimohammadi,Javad Tashkhourian,Sara Mostafapour,Mojtaba Shamsipur
出处
期刊:Analytical Sciences
[Japan Society for Analytical Chemistry]
日期:2023-06-01
卷期号:39 (9): 1455-1464
标识
DOI:10.1007/s44211-023-00360-2
摘要
A rapid, simple, and inexpensive spectrofluorimetric sensor has been developed for the simultaneous determination of methotrexate (MTX) and folic acid (FA) based on their interactions with hollow carbon dots (HCDs). Since the use of folic acid to cope with the toxic side effects of MTX in patients is essential, the simultaneous determination of these two compounds has been interesting. The results showed that MTX could quench the fluorescence of HCDs with a dynamic quenching mechanism. The sensor exhibited a linear concentration range of 1.0 × 10–6–1.9 × 10–4 mol L−1 for MTX and 1.5 × 10–5–9.4 × 10–4 mol L−1 for FA and the obtained detection limits for MTX and FA were 1.6 × 10–7 and 5.0 × 10–7 mol L−1, respectively. The applicability of the method was investigated in the analysis of the urine samples and the partial least squares (PLS) method was used for the simultaneous determination of MTX and FA.
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