EngineeringYarrowia lipolyticaforde novoproduction of tetraacetyl phytosphingosine

To genetically engineer the oleaginous yeast Yarrowia lipolytica for de novo production of tetraacetylphytosphingosine (TAPS), a precursor of phytosphingosine, and optimization of fermentation conditions for high yield. We successfully constructed a TAPS‐producing Y. lipolytica CE3 strain by co‐expression of Wickerhamomyces ciferrii‐derived acetyl transferases, Sli1p and Atf2p. Next, we optimized several environmental factors including temperature, initial pH and C/N ratio for TAPS production in a shake culture. Deletion of LCB4 in CE3 strain increased the volumetric TAPS titre and cell‐specific yield to 142·1 ± 10·7 mgTAPS l−1 and 3·08 ± 0·11 mgTAPS gDCW−1, respectively, in a shake flask culture incubated for 120 h at 28°C with glycerol as the carbon source. Finally, we developed a 5‐l fed‐batch process with NaOH‐mediated pH control and olive oil as a carbon source, exhibiting 650 ± 24 mgTAPS l−1 of TAPS production within 56 h of the fermentation. The introduction of codon‐optimized Sli1p and Atf2p, deletion of LCB4 gene and sexual hybridization, accompanied by specific fermentation conditions, enhanced TAPS yield in Y. lipolytica. Our results highlight Y. lipolytica as a promising candidate for the industrial production of TAPS, an important component of cosmetic formulations.