类黄酮
类黄酮生物合成
银杏
银杏
转录组
生物化学
生物
基因
生物合成
糖基转移酶
基因表达
植物
抗氧化剂
作者
Yaqiong Wu,Tongli Wang,Xin Yue,Guibin Wang,Liang Xu
出处
期刊:Molecules
[MDPI AG]
日期:2020-10-20
卷期号:25 (20): 4836-4836
被引量:15
标识
DOI:10.3390/molecules25204836
摘要
The flavonoids in Ginkgo biloba L. (ginkgo) have important medicinal uses due to their antioxidant, antitumor, and blood circulation-promoting effects. However, the genetic mechanisms underlying flavonoid biosynthesis in ginkgo remain elusive. Flavonoid 3′, 5′-hydroxylase (F3′5′H) is an important enzyme in flavonoid synthesis. We detected a novel differentially expressed GbF3′5′H1 gene homologous to the F3′5′H enzyme involved in the flavonoid synthesis pathway through transcriptome sequencing. In this study, we characterized this gene, performed an expression analysis, and heterologously overexpressed GbF3′5′H1 in Populus. Our results showed that GbF3′5′H1 is abundant in the leaf and highly expressed during April. We also found four metabolites closely related to flavonoid biosynthesis. Importantly, the contents of 4′,5-dihydroxy-7-glucosyloxyflavanone, epicatechin, and gallocatechin were significantly higher in transgenic plants than in nontransgenic plants. Our findings revealed that the GbF3′5′H1 gene functions in the biosynthesis of flavonoid-related metabolites, suggesting that GbF3′5′H1 represents a prime candidate for future studies (e.g., gene-editing) aiming to optimize ginkgo flavonoid production, especially that of flavan-3-ols.
科研通智能强力驱动
Strongly Powered by AbleSci AI