生物
生物钟
染色质免疫沉淀
遗传学
基因组
表观遗传学
昼夜节律
基因
计算生物学
发起人
转录因子
DNA微阵列
基因表达
神经科学
作者
Fumiyuki Hatanaka,Chiaki Matsubara,Jihwan Myung,Takashi Yoritaka,Naoko Kamimura,Seiichiro Tsutsumi,Akinori Kanai,Yutaka Suzuki,Paolo Sassone‐Corsi,Hiroyuki Aburatani,Sumio Sugano,Toru Takumi
摘要
Circadian rhythms are common to most organisms and govern much of homeostasis and physiology. Since a significant fraction of the mammalian genome is controlled by the clock machinery, understanding the genome-wide signaling and epigenetic basis of circadian gene expression is essential. BMAL1 is a critical circadian transcription factor that regulates genes via E-box elements in their promoters. We used multiple high-throughput approaches, including chromatin immunoprecipitation-based systematic analyses and DNA microarrays combined with bioinformatics, to generate genome-wide profiles of BMAL1 target genes. We reveal that, in addition to E-boxes, the CCAATG element contributes to elicit robust circadian expression. BMAL1 occupancy is found in more than 150 sites, including all known clock genes. Importantly, a significant proportion of BMAL1 targets include genes that encode central regulators of metabolic processes. The database generated in this study constitutes a useful resource to decipher the network of circadian gene control and its intimate links with several fundamental physiological functions.
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