一氧化氮
荧光
化学
生物物理学
下调和上调
细胞
内生
缺氧(环境)
生物化学
细胞生物学
氧气
生物
基因
物理
有机化学
量子力学
作者
Yanjun Pan,Xiaoxiao Chen,Lei Dong,Ning Shao,Li‐Ya Niu,Qing‐Zheng Yang
标识
DOI:10.1016/j.cclet.2021.06.024
摘要
Evaluating the correlation between hypoxia inducible factor 1 (HIF-1) and nitric oxide (NO) generated under hypoxia is of great significance. In this work, we developed a fluorescent probe for the monitor of HIF-1 activity influenced by NO under hypoxia in hepatoma cells with dual-targeting for hepatocyte and lipid droplet (LD). The probe shows excellent selectivity to NO and high sensitivity with 6000-fold fluorescence enhancement. Live cell imaging experiments revealed the probe's capability of imaging exogenous and endogenous NO with specific in LDs of HepG2 cells. For cells under hypoxia, HIF-1 induced LD level is observed to correlate with NO level. This work provides the in-situ visualization of NO-dependent HIF-1 upregulation through LD accumulation.
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