胶体金
费斯特共振能量转移
适体
组合化学
表面等离子共振
肽
荧光
纳米技术
蛋白质-蛋白质相互作用
作者
Zheng-Yuan Ma,Yi-Fan Ruan,Fei Xu,Wei-Wei Zhao,Jing-Juan Xu,Hong-Yuan Chen
标识
DOI:10.1021/acs.analchem.6b00012
摘要
In this work, we present a novel energy-transfer (ET)-based photoelectrochemical (PEC) probing of DNA–protein interactions, which associates intimately with many important intracellular processes in transcriptional regulatory networks. Specifically, Au nanoparticles (NPs) were confined onto the CdS quantum dots (QDs) functionalized PEC surface by the formation of duplex DNA, the subsequent binding of the TATA binding protein (TBP) and the resulting distortion of the Au NPs capped DNA sequence could adjust the interparticle distance and thereby modulate the PEC performance of CdS QDs through the ET process between the CdS QDs and Au NPs. Using the duplex DNA sequence as a rigid spacer, the relationship between the photocurrent quenching effect and the spacing distance was also studied and some experimental conditions were optimized, on the basis of which a novel ET-based PEC TBP biosensor was realized with high sensitivity and selectivity.
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