油酸
CYP2C8
细胞色素P450
CYP3A型
肝病
生物标志物
新陈代谢
内科学
肝功能
生物化学
脂肪酸
化学
脂肪肝
内分泌学
生物
医学
疾病
CYP3A4型
作者
Thomas Thum,Sándor Bátkai,Philipp G. Malinski,Thomas Becker,Iris Mevius,Jürgen Klempnauer,H. Meyer,Jürgen C. Frölich,Jürgen Borlak,Dimitrios Tsikas
标识
DOI:10.1111/j.1478-3231.2010.02310.x
摘要
Background: Oleic acid is a major systemically circulating fatty acid in humans with atheroprotective and immunomodulatory properties. As of today, the contribution of individual cytochrome P450 (CYP) mono-oxygenases to the epoxidation of this fatty acid is unknown. Furthermore, the extent of the oleic acid oxidation product cis-9,10-epoxyoctadecanoic acid (cis-EODA) in humans and its plasma levels in patients with impaired liver function are not known. Patients and methods: We studied cis-EODA in plasma of patients suffering from chronic liver diseases, a condition that often displays impaired liver CYP enzyme activities. Fifteen CYP mono-oxygenases were investigated in vitro as a potential source of cis-EODA. Results: Strikingly, plasma levels of cis-EODA were significantly repressed (P<0.0005) when patients with liver impairment (n=16) were compared with healthy subjects (n=14). Production of cis-EODA was catalysed by CYP in the following order: 2C8, 2C9, 2C19, 3A4, 1A2 and CYP3A7. Conclusion: cis-EODA plasma concentrations are decreased in hepatic disease with impaired liver function. Oleic acid is primarily oxidized to oleic acid oxide (cis-EODA) by CYP2C and CYP3A mono-oxygenases. The liver is the major organ responsible for the oxidation of oleic acid to cis-EODA, and thus, cis-EODA may be a suitable biomarker to assess liver function.
科研通智能强力驱动
Strongly Powered by AbleSci AI