DPPH
化学
异黄酮素
大豆黄酮
清除
染料木素
超氧化物
羟基自由基
生物杀虫素A
抗氧化剂
有机化学
生物化学
酶
医学
内科学
作者
Xiaolin Liang,Xiuling Wang,Zhuang Li,Qing‐Hong Hao,Shiying Wang
摘要
Free radical-scavenging activity of isoflavones and some isoflavone metabolites have been described previously, but the results are inconsistent. The objective of the present study was to find out the pivotal factors that influence an accurate detection of both superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. We here showed for the first time that organic solvents, including methanol, ethanol and acetone, were of strong superoxide radical-scavenging activity at concentrations down to 0.1% (v/v), however, no such activity was observed with acetonitrile at concentrations up to 2.0% (v/v). In DPPH assay, we found that the DPPH radical-scavenging ratio increased together with the extended reaction time. Based on our findings, improved in vitro assays for the detection of radical-scavenging activity of both isoflavones (daidzein and genistein) and isoflavone metabolites, including dihydrodaidzein (DHD), dihydrogenistein (DHG), and O-desmethylangolensin (O-Dma), were established.
科研通智能强力驱动
Strongly Powered by AbleSci AI