单克隆抗体
肌钙蛋白复合物
肌钙蛋白I
化学
分子生物学
单克隆
抗体
抗原
肌钙蛋白
生物
医学
内科学
免疫学
心肌梗塞
作者
M. F. M. Fathil,M. K. Md Arshad,Subash C. B. Gopinath,R. Adzhri,A. Rahim Ruslinda,U. Hashim
出处
期刊:Nucleation and Atmospheric Aerosols
日期:2017-01-01
被引量:3
摘要
This paper presents preparation and characterization of conventional enzyme-linked immunosorbent assay (ELISA) for cardiac troponin detection to determine the selectivity of the cardiac troponin monoclonal antibodies. Monoclonal antibodies, used to capture and bind the targets in this experiment, are cTnI monoclonal antibody (MAb-cTnI) and cTnT monoclonal antibody (MAb-cTnT), while both cardiac troponin I (cTnI) and T (cTnT) are used as targets. ELISA is performed inside two microtiter plates for MAb-cTnI and MAb-cTnT. For each plate, monoclonal antibodies are tested by various concentrations of cTnI and cTnT ranging from 0-6400 µg/l. The binding selectivity and level of detection between monoclonal antibodies and antigen are determined through visual observation based on the color change inside each well on the plate. ELISA reader is further used to quantitatively measured the optical density of the color changes, thus produced more accurate reading. The results from this experiment are utilized to justify the use of these monoclonal antibodies as bio-receptors for cardiac troponin detection by using field-effect transistor (FET)-based biosensors coupled with substrate-gate in the future.
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