Comparative Analysis of Automatic Fecal Analyzer versus Direct Wet Smear Microscopy for Detecting Parasitic Infections in Stool Samples

粪便 显微镜 微生物学 生物 医学 病理
作者
Yujing Yang,Qianyun Deng,Chun-Miao Wang,F Wang,Caiping Gong,Xue Jia,Ziqing Deng,Rongchun Huang,Guanghua Li,Yunhu Zhao
出处
期刊:Journal of Visualized Experiments [MyJOVE]
卷期号: (218)
标识
DOI:10.3791/67706
摘要

With socio-economic development, the prevalence of intestinal parasitic diseases has significantly decreased year by year. However, parasitic infections remain a major public health issue globally, particularly in developing countries and regions. Timely diagnosis and treatment are crucial for controlling the spread of these diseases. The traditional Direct Wet Smear Microscopy method, while widely used, is labor-intensive, prone to contamination, and dependent on the skills of the technician. This paper introduces an Automatic Fecal Analyzer, which automates the stool sample processing, offering advantages over the traditional Direct Wet Smear Microscopy method, such as ease of operation, rapid detection, a clean and hygienic working environment, and high sensitivity and specificity, thus enhancing diagnostic efficiency and accuracy. We compared three different methods for fecal analysis: direct wet smear microscopy method, automatic fecal analyzer (AI report), and automatic fecal analyzer (user audit). The AI report uses automated image analysis and machine learning algorithms to identify components like parasites and eggs in fecal samples. This method can process a large number of samples quickly, increasing efficiency. The User Audit also uses an automatic fecal analyzer but includes an additional step of user audit. Experienced technicians review the AI report to enhance the accuracy and reliability of the results.The analyzer demonstrated a sensitivity of 84.31% for AI report and 94.12% for user audits, along with a specificity of 98.71% for AI reports and 99.69% for user audits, making it an invaluable tool for the clinical diagnosis and treatment of parasitic infections.

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