生物
精子无力症
精子活力
精子
微管
不育
男性不育
人类受精
鞭毛
运动性
遗传学
细胞生物学
基因
怀孕
作者
Lunni Zhou,Haobin Liu,Siyu Liu,Xiaoyu Yang,Yaping Dong,Yun Zu Pan,Xiao Zhang,Baodong Zheng,Yi Sun,Pengyu Huang,Xixi Zhang,Haixia Jin,Rui Sun,Shan Feng,Yichao Zhu,Mingxi Liu,Miao Gui,Jianping Wu
出处
期刊:Cell
[Elsevier]
日期:2023-06-01
卷期号:186 (13): 2897-2910.e19
被引量:15
标识
DOI:10.1016/j.cell.2023.05.009
摘要
Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility.
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