纳米载体
适体
阿霉素
体内
化学
癌细胞
药物输送
细胞毒性
透明质酸
靶向治疗
MTT法
靶向给药
流式细胞术
生物化学
体外
癌症研究
药理学
分子生物学
癌症
医学
生物
化疗
内科学
有机化学
生物技术
解剖
作者
Youkabed Kazemi,Sadegh Dehghani,Fatemeh Soltani,Khalil Abnous,Mona Alibolandi,Seyed Mohammad Taghdisi,Mohammad Ramezani
标识
DOI:10.1016/j.nano.2022.102588
摘要
Nanomaterial-based drug delivery has opened new horizons in cancer therapy. This study aimed to investigate the in vitro and in vivo anti-cancer effects of a hyaluronic acid (HA)-targeted nanocarrier based on hollow silica nanoparticles (HSNPs), gated with peptide nucleic acid (PNA) and ATP aptamer (ATPApt) and loaded with doxorubicin (DOX). After formulation of a smart drug delivery nanosystem (HSNPs/DOX/ATPApt/PNA/HA), drug release, cytotoxicity, uptake, and in vivo anti-tumor properties were studied. Drug release test showed the controlled release of encapsulated DOX in response to ATP content. MTT and flow cytometry indicated that HA could improve both cytotoxicity and cellular uptake of the formulation. Moreover, HA-targeted formulation enhanced both the survival rate and tumor inhibition in the tumor-bearing mice compared with free DOX (P < 0.05). Our findings confirmed that HA-targeted nanoformulation, gated with PNA/aptamer and loaded with DOX can provide a novel therapeutic platform with great potential for cancer therapy.
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