牙周膜干细胞
碱性成纤维细胞生长因子
牙周纤维
细胞生物学
化学
细胞分化
间充质干细胞
成纤维细胞生长因子
碱性磷酸酶
生物
生长因子
生物化学
医学
牙科
受体
酶
基因
作者
Yangfan Li,Fenglin Yu,Liu Yang,Liang Qian,Yadong Huang,Qi Xiang,Qihao Zhang,Zhijian Su,Yan Yang,Zhao Yue-ping
摘要
Periodontal ligament stem cells (PDLSCs) play an essential role in periodontal tissue repair. Basic fibroblast growth factor (bFGF) has been used in the clinical treatment of periodontal disease. However, studies have shown that bFGF inhibits the osteogenic differentiation of PDLSCs, which is not conducive to alveolar bone repair. Sulfonated chitosan oligosaccharide (SCOS), a heparan-like compound, can maintain the conformation of bFGF and promote its proliferation activity. This study investigated the effects of bFGF in combination with SCOS on the osteogenic differentiation of hPDLSCs.hPDLSCs were isolated from healthy human periodontal ligament and identified by flow cytometry and immunofluorescence. The affinity between SCOS and bFGF was analyzed by surface plasmon resonance. Changes in osteogenic differentiation by combination of bFGF with SCOS were analyzed by alkaline phosphatase activity assay, Sirius Red staining, and Alizarin Red staining. Expression of genes and proteins was investigated by western blotting and reverse transcription-quantitative PCR.Extracted hPDLSCs were mesenchymal stem cells with pluripotent differentiation potential. SCOS exhibited an affinity for bFGF. bFGF (20 ng/mL) promoted the proliferation of hPDLSCs, but inhibited their osteogenic differentiation. SCOS alleviated the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs.SCOS can reduce the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs. This study provides evidence for the clinical use of bFGF to repair periodontal tissue.
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