Amperometric Glucose Sensor Based on a Polydimethyl Siloxane/Enzyme-Bilayer Membrane

An amperometric glucose-sensing electrode based on the cathodic detection of oxygen consumption during the glucose oxidase (GOx) reaction was prepared as follows. A platinum electrode was modified with polydimethyl-siloxane (PDMS), and a GOx-layer was placed on the PDMS layer. The permselectivity of PMDS made it possible to monitor the oxygen consumption rate without interference from the hydrogen peroxide. The concentration of glucose (0.01-1.2 mM) could be determined from the decrease in the cathodic current at −0.1 V vs. Ag/AgCl, without any interference from uric acid, acetaminophen and L-cysteine.