等色体
荧光原位杂交
生物
12号染色体
生殖细胞肿瘤
染色体
克拉斯
癌症研究
分子生物学
遗传学
核型
化疗
基因
突变
作者
Liang Cheng,Darrell D. Davidson,Rodolfo Montironi,Mingsheng Wang,Antonio López-Beltrán,Timothy A. Masterson,Costantine Albany,Shaobo Zhang
出处
期刊:Methods in molecular biology
日期:2020-08-28
卷期号:: 49-63
被引量:5
标识
DOI:10.1007/978-1-0716-0860-9_4
摘要
Gains of genetic material or internal rearrangements of chromosome 12p, including 12p overrepresentation or isochromosome 12p [i(12p)], are observed in virtually all germ cell tumors (GCT), in all histologic subtypes, and from various body locations. The chromosomal region involved in these alterations contains the growth and survival promoting oncogene KRAS (12p12.1). Gains or rearrangements of 12p characterize GCT from in situ to chemoresistant stages. Fluorescence in situ hybridization (FISH) detection of chromosome 12p anomalies is a sensitive and specific test for the diagnosis of germ cell tumors. Here we provide a detailed protocol for FISH detection of isochromosome 12p and chromosome 12p overrepresentation. The method is helpful for diagnosis of germ cell origin, and for selection of patients who may benefit from cisplatin-based chemotherapy.
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