内生
半胱氨酸
硫化氢
荧光
线粒体
谷胱甘肽
化学
硫黄
生物化学
电泳剂
生物物理学
生物
酶
物理
有机化学
量子力学
催化作用
作者
Yayuan Zheng,Zuo-Hu Chai,Wei Tang,Shuai Yan,Fang Dai,Bo Zhou
标识
DOI:10.1016/j.snb.2020.129343
摘要
Reactive sulfur species (RSS) include sulfur dioxide (SO2) and various biothiols, such as hydrogen sulfide (H2S), glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). These RSS are closely related to each other through complicated symbiotic networks, and play indispensable roles in multiple pathophysiological processes. Due to their similarity in chemical properties, it is challenging for developing a single fluorescent probe to differentiate them simultaneously. Herein we report the first mitochondria-targetable fluorescent probe NIR-NBD for simultaneous discrimination of Cys/Hcy/H2S, GSH, and SO2 from each other. The probe is characterized of the presence of three different types of electrophilic sites, and displays multiple sets of signal pattern in response to Cys/Hcy/ H2S, GSH, and SO2, including red-green for Cys/Hcy/H2S, red for GSH, and green for SO2. Notably, the probe exhibits excellent selectivity, outstanding sensitivity (detection limit = 3.5 nM) and fast response (within 4 min) toward SO2. Furthermore, the probe has been successfully applied for monitoring mitochondrial SO2 and various biothiols, visualizing the endogenous generation of SO2, and probing into the role of biothiols during apoptosis process.
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