压力过载
等压标记
收缩
心力衰竭
心室
肌肉肥大
下调和上调
左心室肥大
蛋白质组学
内科学
化学
医学
细胞生物学
生物
定量蛋白质组学
心肌肥大
生物化学
血压
基因
作者
Dawei Guan,Yan Zhao,Yuan Zhang,Dongqi Tang,Qi Wu
出处
期刊:Protein and Peptide Letters
[Bentham Science]
日期:2016-01-05
卷期号:23 (2): 125-131
被引量:5
标识
DOI:10.2174/0929866523666151125231747
摘要
To identify proteins involved in the molecular mechanisms of pressure overload-induced heart failure, we quantitatively analyzed the left ventricular proteomic profile in a transverse aortic constriction (TAC) mouse model, a well-established model of cardiac hypertrophy and dysfunction. Multidimensional LC-MS/MS in combination with Isobaric Tags for Relative and Absolute Quantification (iTRAQ) labeling were used to analyze cardiac tissue samples and identify proteins that are differentially expressed in the left ventricle of mice that were challenged by TAC through 14 and 28 days. In this study, 1,748 high-quality reference proteins were identified compared with control mice, which showed 64 proteins up-regulated and 68 proteins down-regulated in the left ventricle of TAC mice through 14 days. Meanwhile, 46 proteins were upregulated and 40 proteins were down-regulated in the left ventricle of TAC mice through 28 days. Of all the differentially expressed proteins, we performed GO, pathway analysis and their interactions to the differentially expressed proteins and found that most of the proteins are related to cardiac muscle contraction, energy metabolism, occurrence of diseases and signal transduction. These proteins represent potential novel diagnostic and therapeutic targets for the treatment of pressure overload-induced heart failure.
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