花粉
生物
转基因
基因
格斯报告系统
报告基因
启动子活性
转基因作物
花粉管
植物
发起人
小孢子
遗传学
基因表达
授粉
作者
Ling Chen,Yingjie Miao,Cheng Wang,Peipei Su,Tianheng Li,Rong Wang,Xinglong Hao,Guangxiao Yang,Guangyuan He,Chunbao Gao
标识
DOI:10.1007/s11105-012-0458-y
摘要
PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.
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