不透明度
土壤水分
体内
多孔介质
透明度(行为)
材料科学
根毛
营养物
多孔性
生物物理学
环境科学
化学
计算机科学
土壤科学
生物
生物技术
生态学
生物化学
复合材料
光学
基因
计算机安全
物理
作者
Lin Ma,Yichao Shi,Oskar Siemianowski,Baozong Yuan,Timothy K. Egner,Seyed Vahid Mirnezami,Kara R. Lind,Baskar Ganapathysubramanian,Vincenzo Venditti,Ludovico Cademartiri
标识
DOI:10.1073/pnas.1820334116
摘要
Root phenotypes are increasingly explored as predictors of crop performance but are still challenging to characterize. Media that mimic field conditions (e.g., soil, sand) are opaque to most forms of radiation, while transparent media do not provide field-relevant growing conditions and phenotypes. We describe here a "transparent soil" formed by the spherification of hydrogels of biopolymers. It is specifically designed to support root growth in the presence of air, water, and nutrients, and allows the time-resolved phenotyping of roots in vivo by both photography and microscopy. The roots developed by soybean plants in this medium are significantly more similar to those developed in real soil than those developed in hydroponic conditions and do not show signs of hypoxia. Lastly, we show that the granular nature and tunable properties of these hydrogel beads can be leveraged to investigate the response of roots to gradients in water availability and soil stiffness.
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