明胶
材料科学
3D生物打印
组织工程
生物活性玻璃
生物加工
纳米颗粒
纳米技术
间质细胞
纳米复合材料
介孔二氧化硅
生物医学工程
介孔材料
化学
癌症研究
复合材料
催化作用
生物
医学
生物化学
作者
Hongqin Zhu,Mahshid Monavari,Kai Zheng,Thomas Distler,Liliang Ouyang,Susanne Heid,Zhaorui Jin,Jiankang He,Dichen Li,Aldo R. Boccaccını
出处
期刊:Small
[Wiley]
日期:2022-02-01
卷期号:18 (12)
被引量:49
标识
DOI:10.1002/smll.202104996
摘要
Bioprinting has seen significant progress in recent years for the fabrication of bionic tissues with high complexity. However, it remains challenging to develop cell-laden bioinks exhibiting superior physiochemical properties and bio-functionality. In this study, a multifunctional nanocomposite bioink is developed based on amine-functionalized copper (Cu)-doped mesoporous bioactive glass nanoparticles (ACuMBGNs) and a hydrogel formulation relying on dynamic covalent chemistry composed of alginate dialdehyde (oxidized alginate) and gelatin, with favorable rheological properties, improved shape fidelity, and structural stability for extrusion-based bioprinting. The reversible dynamic microenvironment in combination with the impact of cell-adhesive ligands introduced by aminated particles enables the rapid spreading (within 3 days) and high survival (>90%) of embedded human osteosarcoma cells and immortalized mouse bone marrow-derived stroma cells. Osteogenic differentiation of primary mouse bone marrow stromal stem cells (BMSCs) and angiogenesis are promoted in the bioprinted alginate dialdehyde-gelatin (ADA-GEL or AG)-ACuMBGN scaffolds without additional growth factors in vitro, which is likely due to ion stimulation from the incorporated nanoparticles and possibly due to cell mechanosensing in the dynamic matrix. In conclusion, it is envisioned that these nanocomposite bioinks can serve as promising platforms for bioprinting complex 3D matrix environments providing superior physiochemical and biological performance for bone tissue engineering.
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