离体
医学
急性胰腺炎
脂肪酸
乙酯
胰腺炎
体内
药理学
内科学
生物化学
体外
化学
生物
有机化学
生物技术
作者
Aparna Jakkampudi,Ramaiah Jangala,Ratnakar Reddy,Balkumar Reddy,Govind Pratap Rao,R. Pradeep,D. Nageshwar Reddy,Rupjyoti Talukdar
出处
期刊:Pancreatology
[Elsevier]
日期:2020-10-12
卷期号:20 (8): 1620-1630
被引量:4
标识
DOI:10.1016/j.pan.2020.10.027
摘要
Abstract Background & aim Fatty acid ethyl esters (FAEEs), are produced by non-oxidative alcohol metabolism and can cause acinar cell damage and subsequent acute pancreatitis in rodent models. Even though experimental studies have elucidated the FAEE mediated early intra-acinar events, these mechanisms have not been well studied in humans. In the present study, we evaluate the early intra-acinar events and inflammatory response in human pancreatic acinar tissues and cells in an ex-vivo model. Methods Experiments were conducted using normal human pancreatic tissues exposed to FAEE. Subcellular fractionation was performed on tissue homogenates and trypsin and cathepsin B activities were estimated in these fractions. Acinar cell injury was evaluated by histology and immunohistochemistry. Cytokine release from exposed acinar cells was evaluated by performing Immuno-fluorescence. Serum was collected from patients with AP within the first 72 h of symptom onset for cytokine estimation using FACS. Results We observed significant trypsin activation and acinar cell injury in FAEE treated tissue. Cathepsin B was redistributed from lysosomal to zymogen compartment at 30 min of FAEE exposure. IHC results indicated the presence of apoptosis in pancreatic tissue at 1 & 2hrs of FAEE exposure. We also observed a time dependent increase in secretion of cytokines IL-6, IL-8, TNF-α from FAEE treated acinar tissue. There was also a significant elevation in plasma cytokines in patents with alcohol associated AP within 72 h of symptom onset. Conclusion Our data suggest that alcohol metabolites can cause acute acinar cell damage and subsequent cytokine release which could eventually culminant in SIRS.
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