清脆的
生物
基因组编辑
类有机物
基因组工程
干细胞
计算生物学
人诱导多能干细胞
诱导多能干细胞
体细胞
基因组
胚胎干细胞
遗传学
基因
作者
Delilah Hendriks,Hans Clevers,Benedetta Artegiani
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2020-11-01
卷期号:27 (5): 705-731
被引量:92
标识
DOI:10.1016/j.stem.2020.10.014
摘要
CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.
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