Tackling the Selectivity Dilemma of Benzopyrylium–Coumarin Dyes in Fluorescence Sensing of HClO and SO2

Benzopyrylium-coumarin fluorescent probes for sensing hypochlorous acid (HClO) or sulfur dioxide (SO₂) are unable to distinguish between HClO and SO₂ because the two compounds can react with the 4-position of benzopyrylium-coumarin dyes through the nucleophilic attack. In the current work, we introduced a phenoxazine moiety to the benzopyrylium-coumarin dye to synthesize a new fluorescent probe PBC1, which can dually sense HClO and SO₂ and generate distinct fluorescence signals with rapid response time and high sensitivity and selectivity. Moreover, probe PBC1 was also successfully utilized to detect intracellular HClO and SO₂ in HeLa cells and zebrafish.