自愈水凝胶
薄壁组织
创伤性脑损伤
再生(生物学)
神经干细胞
小胶质细胞
脑组织
组织工程
生物医学工程
移植
材料科学
化学
医学
细胞生物学
病理
炎症
干细胞
生物
外科
免疫学
精神科
高分子化学
作者
Satoshi Tanikawa,Yuki Ebisu,Tomáš Sedlačík,Shingo Semba,Takayuki Nonoyama,Akira Hirota,Taiga Takahashi,Kazushi Yamaguchi,Masamichi Imajo,Harunosuke Kato,Takuya Nishimura,Zen‐ichi Tanei,Masumi Tsuda,Tomomi Nemoto,Jian Ping Gong,Shinya Tanaka
标识
DOI:10.1101/2022.02.16.480448
摘要
Abstract Neural regeneration is extremely difficult to achieve. In traumatic brain injuries, the loss of brain parenchyma volume hinders neural regeneration. In this study, neuronal tissue engineering was performed by using electrically charged hydrogels composed of cationic and anionic monomers in a 1:1 ratio (C1A1 hydrogel), which served as an effective scaffold for the attachment of neural stem cells (NSCs). In the 3D environment of porous C1A1 hydrogels engineered by the cryogelation technique, NSCs differentiated into neuroglial cells. The C1A1 porous hydrogel was implanted into brain defects in a mouse traumatic damage model. The VEGF-immersed C1A1 porous hydrogel promoted host-derived vascular network formation together with the infiltration of macrophages/microglia and astrocytes into the gel. Furthermore, the stepwise transplantation of GFP-labeled NSCs supported differentiation to glial and neuronal cells. Therefore, this two-step method for neural regeneration may become a new approach for therapeutic brain tissue reconstruction after brain damage in the future. One Sentence Summary Brain tissue reconstruction using charged hydrogel and stepwise NCS injection
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