显微镜
磁性纳米粒子
病理
材料科学
H&E染色
核磁共振
磁共振成像
钻石
磁力显微镜
生物医学工程
染色
纳米技术
医学
纳米颗粒
放射科
磁场
物理
磁化
复合材料
量子力学
作者
Sanyou Chen,Wan-He Li,Xiaohu Zheng,Pei Yu,Pengfei Wang,Ziting Sun,Yao Xu,Defeng Jiao,Xiangyu Ye,Mingcheng Cai,Minfen Shen,Mengqi Wang,Qi Zhang,Fei Kong,Ya Wang,Jie He,Haiming Wei,Fazhan Shi,Jiangfeng Du
标识
DOI:10.1073/pnas.2118876119
摘要
Histological imaging is essential for the biomedical research and clinical diagnosis of human cancer. Although optical microscopy provides a standard method, it is a persistent goal to develop new imaging methods for more precise histological examination. Here, we use nitrogen-vacancy centers in diamond as quantum sensors and demonstrate micrometer-resolution immunomagnetic microscopy (IMM) for human tumor tissues. We immunomagnetically labeled cancer biomarkers in tumor tissues with magnetic nanoparticles and imaged them in a 400-nm resolution diamond-based magnetic microscope. There is barely magnetic background in tissues, and the IMM can resist the impact of a light background. The distribution of biomarkers in the high-contrast magnetic images was reconstructed as that of the magnetic moment of magnetic nanoparticles by employing deep-learning algorithms. In the reconstructed magnetic images, the expression intensity of the biomarkers was quantified with the absolute magnetic signal. The IMM has excellent signal stability, and the magnetic signal in our samples had not changed after more than 1.5 y under ambient conditions. Furthermore, we realized multimodal imaging of tumor tissues by combining IMM with hematoxylin-eosin staining, immunohistochemistry, or immunofluorescence microscopy in the same tissue section. Overall, our study provides a different histological method for both molecular mechanism research and accurate diagnosis of human cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI