硫黄素
胶束
纤维
化学
淀粉样蛋白(真菌学)
荧光
生物物理学
苯并噻唑
刚果红
水溶液
生物化学
阿尔茨海默病
有机化学
无机化学
生物
病理
吸附
物理
医学
量子力学
疾病
作者
Rohit Khurana,Chris L. Coleman,Cristian Ionescu-Zanetti,Sue A. Carter,Vinay Krishna,Rajesh K. Grover,Raja Roy,Shashi Singh
标识
DOI:10.1016/j.jsb.2005.06.006
摘要
Thioflavin T is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, thioflavin T was found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay ( approximately 10-20 microM). Specific conductivity changes were measured at varying concentration of thioflavin T and the critical micellar concentration was calculated to be 4.0+/-0.5 microM. Interestingly, changes in the fluorescence excitation and emission of thioflavin T were also dependent on the micelle formation. The thioflavin T micelles of 3 nm diameter were directly visualized using atomic force microscopy, and bound thioflavin T micelles were observed along the fibril length for representative fibrils. Increasing concentration of thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles were disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3. This suggests that positive charge on the thioflavin T molecule has a role in its micelle formation that then bind the amyloid fibrils. Our data suggests that the micelles of thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission.
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