炎症
医学
移植
体内
脂肪组织
纤维化
体外
药理学
癌症研究
免疫学
生物化学
病理
生物
内科学
生物技术
作者
Jiaming Sun,Chia-Kang Ho,Ya Gao,Chio-Hou Chong,Yangdan Liu,Yuxin Liu,Duo Zheng,Yifan Zhang,Yutong Lu
出处
期刊:Aesthetic Surgery Journal
[Oxford University Press]
日期:2022-12-20
卷期号:43 (5): NP372-NP390
被引量:1
摘要
Abstract Background Autologous fat grafting is a common method for soft tissue defect repair. However, the high absorption rate of transplanted fat is currently a bottleneck in the process. Excessive inflammation is one of the main reasons for poor fat transplantation. Salvianolic acid B (Sal-B) is a herbal medicine that shows promise for improving the effectiveness of fat transplantation. Objective The aim of this study was to improve fat graft survival by injecting Sal-B into fat grafts locally. Methods In vivo, 0.2 mL of Coleman fat was transplanted into nude mice along with Sal-B. The grafts were evaluated by histologic analysis at 2, 4, and 12 weeks posttransplantation and by microcomputed tomography at 4 weeks posttransplantation. In vitro ribonucleic acid sequencing, cell proliferation assays, anti-inflammatory activity assays, molecular docking studies, and kinase activity assays were performed in RAW264.7 cells to detect the potential mechanism. Results Sal-B significantly improved fat graft survival and attenuated adipose tissue fibrosis and inflammation. Sal-B also inhibited the polarization of M1 macrophages in fat grafts. In vitro, Sal-B inhibited the proliferation and activation of inflammatory pathways in RAW264.7 cells. In addition, Sal-B had an inhibitory effect on NF-κB (nuclear factor κ light polypeptide gene enhancer in B cells) signaling. This bioactivity of Sal-B may result from its selective binding to the kinase domain of the inhibitor of NF-κB kinase subunit β. Conclusions Sal-B could serve as a promising agent for improving the effect of fat transplantation by inhibiting the polarization of M1 macrophages through NF-κB signaling.
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