壳聚糖
石墨烯
碱性磷酸酶
成骨细胞
氧化物
粘附
化学
细胞粘附
生物相容性
材料科学
化学工程
生物物理学
纳米技术
体外
生物化学
有机化学
生物
酶
工程类
作者
Sonia How Ming Wong,Siew Shee Lim,Timm Joyce Tiong,Pau Loke Show,Hayyiratul Fatimah Mohd Zaid,Hwei‐San Loh
摘要
An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt % of 0, 2, 4, and 6 into chitosan matrix via direct blending of chitosan solution and graphene oxide, freezing, and freeze drying. Cell fixation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase colorimetric assays were conducted to assess cell adhesion, proliferation, and early differentiation of MG63 on chitosan–graphene oxide scaffolds respectively. The presence of alkaline phosphatase, an early osteoblast differentiation marker, was further detected in chitosan–graphene oxide scaffolds using western blot. These results strongly supported that chitosan scaffolds loaded with graphene oxide at 2 wt % mediated cell adhesion, proliferation, and early differentiation due to the presence of oxygen-containing functional groups of graphene oxide. Therefore, chitosan scaffolds loaded with graphene oxide at 2 wt % showed the potential to be developed into functional bone scaffolds.
科研通智能强力驱动
Strongly Powered by AbleSci AI