指数富集配体系统进化
寡核苷酸
计算生物学
化学
适体
组合化学
分子生物学
生物
生物化学
DNA
基因
核糖核酸
作者
Daniel Drolet,Lotus Moon-McDermott,Timothy S Romig
摘要
The recent development of in vitro methods to select high-affinity ligands by combinatorial chemistry methodologies promises unique and theoretically unlimited supplies of novel therapeutic and diagnostic reagents. One such combinatorial chemistry process, systematic evolution of ligands by exponential enrichment (SELEX), allows rapid identification, from large random sequence pools, of the few oligonucleotide sequences that bind to a desired target molecule with high affinity and specificity. We describe an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide. This assay demonstrates that these oligonucleotides can be effective and useful analytical reagents.
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