沟道视紫红质
光遗传学
毫秒
计算机科学
材料科学
光电子学
电生理学
二极管
生物系统
神经科学
光记录
多电极阵列
生物神经网络
发光二极管
微电极
化学
物理
生物
电信
物理化学
机器学习
天文
电极
作者
Nir Grossman,V. Poher,Matthew Grubb,Gordon T. Kennedy,Konstantin Nikolić,Brian McGovern,Rolando Berlinguer‐Palmini,Zheng Gong,Emmanuel M. Drakakis,Mark A. A. Neil,Martin D. Dawson,Juan Burrone,Patrick Degenaar
出处
期刊:Journal of Neural Engineering
[IOP Publishing]
日期:2010-01-14
卷期号:7 (1): 016004-016004
被引量:224
标识
DOI:10.1088/1741-2560/7/1/016004
摘要
Studying neuronal processes such as synaptic summation, dendritic physiology and neural network dynamics requires complex spatiotemporal control over neuronal activities. The recent development of neural photosensitization tools, such as channelrhodopsin-2 (ChR2), offers new opportunities for non-invasive, flexible and cell-specific neuronal stimulation. Previously, complex spatiotemporal control of photosensitized neurons has been limited by the lack of appropriate optical devices which can provide 2D stimulation with sufficient irradiance. Here we present a simple and powerful solution that is based on an array of high-power micro light-emitting diodes (micro-LEDs) that can generate arbitrary optical excitation patterns on a neuronal sample with micrometre and millisecond resolution. We first describe the design and fabrication of the system and characterize its capabilities. We then demonstrate its capacity to elicit precise electrophysiological responses in cultured and slice neurons expressing ChR2.
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